Figure 5

Transcript level of HpGSH2. RT-PCR was performed on RNA samples using HpGSH2 primers from cultures of wild type, mcGSH2 and MOXp-GSH2 recombinant strains shifted for 6 hours from glucose medium to glucose (Glc), methanol (Meth) or with no carbon added (-C) media. As loading control transcript levels of actin (ACT1) were analysed.