Figure 1

Characterization of purified recombinant Pf MSP1 ₁₉ -specific human IgA. 5 μg purified recombinant Abs were run under (A) non-denaturing (native) conditions on 6% acrylamide Tris-glycine gels (Novex) or under (B) denaturing reducing conditions on NuPAGE 4-12% Bis-Tris gradient SDS gels (Novex). In the former, anti-NIP IgA2 (AbD Serotec, Kidlington, UK) and anti-NIP IgM (AbD Serotec) were included as polymeric controls. In the latter, gels were either stained with Coomassie or transferred to nitrocellulose and probed with anti-human IgA or anti-mouse IgG-Fc reagents, and lanes containing both IgA and the anti-FcαRI mAb 2H8 were included. The IgA is recognized by anti-human IgA-specific reagents but not by anti-mouse IgG-Fc reactive Abs that only recognize mAb 2H8.