Figure 9From: Site-specific modification of ED-B-targeting antibody using intein-fusion technologyBiotinylation of L19 IgG-DnaE N by PTS. Trans-splicing was initiated with 50 μM DnaEC-CFNK-Biotin in the presence of 5 mM DTT. After purification by His tag affinity chromatography, the product was analyzed by reducing SDS-PAGE and Western blotting. Staining with Streptavidin-Alkaline-Phosphatase revealed specific biotin derivatization of the heavy chain.Back to article page