Figure 6From: Site-specific modification of ED-B-targeting antibody using intein-fusion technologyBiotinylation of L19 IgG-GyrA after in vitro cleavage. EPL was performed with 50 mM Mesna and 5 mM CysLys-Biotin, the product was purified by His tag affinity chromatography and analyzed by reducing SDS-PAGE. Western blotting with Streptavidin-Alkaline-Phosphatase revealed specific biotin derivatization of the heavy chain.Back to article page