Figure 3From: Site-specific modification of ED-B-targeting antibody using intein-fusion technologyL19 IgG-GyrA after EPL. Analysis by reducing SDS-PAGE (Coomassie stained). Purified fusion protein was treated with 50 mM Mesna and 5 mM cysteine for 22h at RT. After dialysis, cleaved L19 IgG was purified by His tag affinity chromatography (1: L19 IgG-GyrA before cleavage, 2: after cleavage, before purification, 3: after purification, HC: heavy chain, LC: light chain).Back to article page