Figure 2From: Site-specific modification of ED-B-targeting antibody using intein-fusion technologyIn vivo cleavage. Mammalian expression medium was conducted to His tag affinity chromatography and the eluate was analyzed by reducing SDS-PAGE (Coomassie stained). 10-15% of L19 IgG-GyrA is cleaved during expression. L19 IgG-DnaEN shows no in vivo cleavage (HC: heavy chain, LC: light chain).Back to article page