GST transfer to L19 IgG-DnaE
by PTS. Intein activation was performed with 5 mM DTT and 50 μM DnaEC-GST and analyzed by reducing SDS-PAGE. (A) Coomassie staining revealed HC-GST (~80 kDa) as main product and HC as side product. 1: DnaEC-GST. 2: L19 IgG-DnaEN. 3: L19 IgG-DnaEN + DnaEC-GST. (B) Western blotting with anti-GST antibody confirmed the transfer of GST onto the heavy chain of L19 IgG (HC: heavy chain, LC: light chain).