Figure 8
From: Expression of tung tree diacylglycerol acyltransferase 1 in E. coli
PreScission protease digestion of recombinant DGAT1. The Ni-NTA affinity-purified rDGAT1 was digested with PreScission protease followed by centrifugation at 10,000g. Equivalent amounts of protein in the undigested, the supernatant and the pellet of the digestion mixtures were separated by 4-20% SDS-PAGE and detected with anti-MBP-mTTP antibodies. The full-length rDGAT1 and the released MBP are marked with arrows.