Plasmid map used for the construction of an E. coli expression vector and primer sequences used for PCR-amplification of the DGAT1 insert. (A) Diagram of the expression plasmid pMBP-DGAT1-His. Plasmid pMBP-hTTP  was used to express full-length DGAT1 in E. coli. Tung DGAT1 DNA was subcloned as described in the Materials and Methods section. (B) Primers for construction of the E. coli expression plasmid. The sequences for restriction enzyme digestion sites are underlined. DGAT1 forward primer and reverse primer have sequences coding for a PreScission protease digestion site and 6 histidine residues, respectively.