Table 2 Oligonucleotides used in this study
From: T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis
Name | Sequence | Properties |
|---|---|---|
Universal miRNA Cloning Linker (17-mer DNA adapter) | CTGTAGGCACCATCAAT | 5'-rApp 3'-NH2 |
5'-FAM labeled 31-mer RNA | rArGrUrCrGrUrArGrCrCrUrUrUrArUrCrCrGrArGrArUrUrCrArGrCrArArUrA | 5'-FAM |
22-mer DNA adapter | TCGTATGCCGTCTTCTGCTTGT | 5'-PO4 3'-NH2 |
5'PO4 21-mer | rArGrCrArGrUrGrGrCrUrGrGrUrUrGrArGrArUrUrU | 5'-PO4 |
28-mer RNA | rArCrArArGrCrArGrArArGrArCrGrGrCrArUrArCrGrArUrArUrUrGrC | 5'-PO4 3'-NH2 |