T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis

BMC Biotechnology

Table 1 Differences in mean RNA substrate ligated by T4 Rnl2tr mutants over time.

Time (h)	Rnl2tr + MBP	K227Q	R55K	K225R	R55K K227Q	K225R K227Q	R55K K225R K227Q
1	NS	*	NS	**	NS	NS	NS
2	NS	***	NS	***	NS	NS	**
3	NS	***	NS	***	NS	**	***
6	NS	***	NS	***	NS	**	***
9	NS	***	NS	***	NS	NS	***
12	NS	**	NS	***	NS	NS	**
24	NS	NS	NS	***	NS	NS	NS

Mean levels of RNA substrate ligated by T4 Rnl2tr mutants that are significantly different are lower than the corresponding WT T4 Rnl2tr values (see Fig 6). NS = no significant difference detected, * = means differ p < 0.05, ** = means differ p < 0.01, *** = means differ p < 0.001 as determined by two-way ANOVA followed by Bonferroni post-tests for comparison to T4 Rnl2tr.

ISSN: 1472-6750

Time (h)	Rnl2tr + MBP	K227Q	R55K	K225R	R55K K227Q	K225R K227Q	R55K K225R K227Q
1	NS	*	NS	**	NS	NS	NS
2	NS	***	NS	***	NS	NS	**
3	NS	***	NS	***	NS	**	***
6	NS	***	NS	***	NS	**	***
9	NS	***	NS	***	NS	NS	***
12	NS	**	NS	***	NS	NS	**
24	NS	NS	NS	***	NS	NS	NS

Time (h)	Rnl2tr + MBP	K227Q	R55K	K225R	R55K K227Q	K225R K227Q	R55K K225R K227Q
1	NS	*	NS	**	NS	NS	NS
2	NS	***	NS	***	NS	NS	**
3	NS	***	NS	***	NS	**	***
6	NS	***	NS	***	NS	**	***
9	NS	***	NS	***	NS	NS	***
12	NS	**	NS	***	NS	NS	**
24	NS	NS	NS	***	NS	NS	NS

Time (h)	Rnl2tr + MBP	K227Q	R55K	K225R	R55K K227Q	K225R K227Q	R55K K225R K227Q
1	NS	*	NS	**	NS	NS	NS
2	NS	***	NS	***	NS	NS	**
3	NS	***	NS	***	NS	**	***
6	NS	***	NS	***	NS	**	***
9	NS	***	NS	***	NS	NS	***
12	NS	**	NS	***	NS	NS	**
24	NS	NS	NS	***	NS	NS	NS