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Table 2 Efficiency of miR-specific qPCR assays with LNA-spiked and DNA primers on pig uterus total RNA

From: Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers

Target Efficiency LNA primers Efficiency DNA primers Difference
let-7a 82% 89% 6.9%
miR-101a 85% 90% 4.9%
miR-103 93% 94% 1.6%
miR-122 95% 95% -0.1%
miR-125b 89% 94% 4.5%
miR-139b-5p 79% 86% 6.4%
miR-150 84% 97% 12.6%
miR-199b-3p 80% 87% 7.1%
miR-20a 88% 86% -2.0%
miR-200b 80% 94% 13.6%
miR-200c 83% 84% 0.2%
miR-21 91% 92% 1.1%
miR-23a 79% 93% 14.1%
miR-23b 81% 87% 6.2%
miR-25 84% 91% 6.7%
miR-26a 88% 96% 8.3%
miR-27a 86% 85% -1.1%
let-7d not specific 102%  
Average 85% 90% 5.4%