MiR-specific qPCR on biological samples with DNA primers. A Amplification curves of 40 uterus samples with the ssc-miR-150 miR-specific qPCR assay. B Melting curve analysis of the same experiment. Melting curve analysis was performed from 55°C to 95°C. C Extrapolation of Cq as function of the log10 of the number of templates for the same experiment as in A was a straight line (R2 = 1.0) with a slope of -3.406 (PCR efficiency = 97%) over 4 log10 dilution of a pool that includes all samples included in the study.