Figure 1From: Specific and sensitive quantitative RT-PCR of miRNAs with DNA primersFlow scheme of miR-specific qPCR. 1. Start with purified RNA containing miRNA. 2. Add poly(A) tail with poly(A) polymerase (PAP). 3. cDNA synthesis with reverse transcriptase (RTase) and an anchored poly(T) primer with a 5' tag. 4. PCR with two tagged primers.Back to article page