Table 2 Summary of plant derived recombinant human collagen I α 1

Tobacco Leaf

P35S (constitutive) + PR-protein SP

proα1(I) ΔNproα1(I)

30 mg/kg powdered plants

N/A

0.53

10

Tobacco Leaf

P35S (constitutive) + PR-protein SP

ΔNproα1(I)

N/A

N/A

N/A

27

Tobacco Leaf

L3 + PR-protein SP

ΔNproα1(I)

0.5-1 mg/kg leaf material

P1287 + Native SP + C. elegans P4Hα/Mouse P4Hβ

8.41

16

Tobacco

rbcS1 (constitutive) + vacuole or apoplast targeting SPs

proα1(I)/proα2(I)

200 mg/kg fresh leaves

P35S (constitutive) + vacuole or apoplast targeting SPs + Human P4Hα/β

7.55

17

Barley P1 cell

Ubi (constitutive) + At chitinase SP + HDEL (ER retention)

proα1(I)

2-9 μg/L cell culture

N/A

N/A

7

Barley Seed

GluB1 (endosperm specific) + At chitinase SP + HDEL (ER retention)

CIα1 45 kD

Below detectable level (CIα1)

45 mg/kg seed (45 kD)

N/A

N/A (CIα1) 2.8 (45 kD)

13

Maize Seed

globulin-1 (embryo specific) + barley α-amylase SP

44 kD

20 mg/kg seed

N/A

2.01

29

Maize Seed

globulin-1 (embryo specific) + barley α-amylase SP

CIα1

3 mg/kg seed

N/A

1.23

11

Maize Seed

globulin-1 (embryo specific) + barley α-amylase SP

CIα1 44 kD

15.9 mg/kg germ (CIα1)

49.6 mg/kg germ (44 kD)

N/A

N/A

12

Maize Seed

globulin-1 (embryo specific) + barley α-amylase SP

CIα1

12 mg/kg seed (CIα1)

4 mg/kg seed (CIα1-OH)

Pubi (constitutive) + Barley α-amylase SP + Human P4Hα/β

18.11

This study