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Table 1 Summary of HRMS analysis on five CIα1 (1057 AA1) samples from maize, Pichia and human

From: Hydroxylation of recombinant human collagen type I alpha 1 in transgenic maize co-expressed with a recombinant human prolyl 4-hydroxylase

 

Pichia

CIα1 (FE291)

Maize

CIα1 (CGB)

Human

CIα1

Maize

CIα1-OH (CGD)

Pichia

CIα1-OH (FE285)

Peptides identified by HRMS (highlighted in yellow, Figure 4)

Total # AA

680

752

620

818

907

Percent HRMS coverage

64.33%

71.14%

58.66%

77.39%

85.81%

Peptide regions identified in all five C1α1 (475 AA) by HRMS (red boxes, Figure 4)

Total # HYP identified by HRMS, in green

2

28

71

86

83

Percent HYP identified by HRMS

0.42%

5.89%

14.95%

18.11%

17.47%

% HYP (by AA analysis)

N/A

1.23%2

10.8%3

N/A

11.54%2

  1. 1sequences presents in all five samples
  2. 2from reference [11]
  3. 3from reference [28]