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Figure 4 | BMC Biotechnology

Figure 4

From: Hydroxylation of recombinant human collagen type I alpha 1 in transgenic maize co-expressed with a recombinant human prolyl 4-hydroxylase

Figure 4

LC-MS/MS analysis of the rCIα1. Full length peptide sequences of 1057 amino acid are listed. Pichia rCIα1, Pichia-derived rCIα1 from strain FE291; Maize rCIα1, maize-derived rCIα1 from line CGB; Human CIα1, gel-isolated CIα1 fragment from commercial collagen (CalBiochem Inc); Maize rCIα1-OH, maize-derived rCIα1 from line CGD; Pichia rCIα1-OH, Pichia-derived rCIα1 from strain FE285. Yellow-highlighted letters: amino acid sequences identified by the Orbitrap; green-highlighted letters: Hyp residues identified by the Orbitrap; red boxes: peptide regions identified in all five samples by the Orbitrap. Black boxes: collagenous triplets Xaa-Pro-Gly with Pro changed to Hyp in Maize rCIα1-OH but not in Maize rCIα1; single underlines: triplets with Pro unchanged in both maize lines; double underlines: triplets with Pro changed to Hyp in both maize lines.

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