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Table 4 ANOVA and Regression Analysis of the Quadratic Model

From: Immobilized Rhizopus oryzaelipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology

Source of variation

Sum of Squares

Degrees of freedom

Mean Square

F-value

p-value Prob > F

Signif

Model

11483.454

14

820.247

10.392

0.0044

a

X1

1.901

1

1.901

0.024

0.8818

 

X2

2336.545

1

2336.545

29.603

0.0016

 

X3

95.976

1

95.976

1.216

0.3124

 

X4

583.453

1

583.453

7.392

0.0347

 

X1 X2

138.198

1

138.198

1.751

0.2340

 

X1 X3

638.138

1

638.138

8.085

0.0294

 

X1 X4

256.492

1

256.492

3.250

0.1215

 

X2 X3

7.742

1

7.742

0.098

0.7647

 

X2 X4

91.839

1

91.839

1.164

0.3222

 

X3 X4

60.006

1

60.006

0.760

0.4168

 

X12

1050.075

1

1050.075

13.304

0.0107

 

X22

319.292

1

319.292

4.045

0.0910

 

X32

1062.246

1

1062.246

13.458

0.0105

 

X42

100.796

1

100.796

1.277

0.3016

 

Residuals

473.577

6

78.929

   

Lack of Fit

339.721

2

169.861

5.076

0.0799

b

Pure Error

133.856

4

33.464

   

Cor Total

11957.031

20

    

R-Squared = 0.960

 
  1. a: indicates significant at the level of 99%
  2. b: indicates insignificant at the level of 99%
  3. Estimated standard deviation is equal to Std. Dev. = √473.577/6 = 8.884