From: Immobilized Rhizopus oryzaelipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology
Factor
Variables
Unit
level
-1
0
1
Temperature
X1
°C
30
40
50
Enzyme Concentration
X2
IU/mL
33.34
166.67
300
Molar ratio (Cetyl alcohol/Palm stearin)
X3
mol/mol
3
5
7
Substrate Concentration
X4
g/mL
0.06
0.2
0.34