Table 1 Experimental conditions of the composite design and the corresponding experimental response
Experimental conditions | Response | |||||
|---|---|---|---|---|---|---|
Run | Temperature (°C) | Enzyme Concentration (IU/mL) | Molar ratio (mol/mol) | Substrate Concentration (g/mL) | Actual yield (%) | Predicted yield (%) |
1 | 50 (+1) | 300 (+1) | 7 (+1) | 0.06 (-1) | 99.51 | 96.52 |
2 | 50 (+1) | 300 (+1) | 3 (-1) | 0.06 (-1) | 73.15 | 75.97 |
3 | 50 (+1) | 33.34 (-1) | 7 (+1) | 0.34 (+1) | 44.41 | 41.42 |
4 | 30 (-1) | 300 (+1) | 3 (-1) | 0.34 (+1) | 52.86 | 55.68 |
5 | 50 (+1) | 33.34 (-1) | 3 (-1) | 0.34 (+1) | 11.03 | 13.85 |
6 | 30 (-1) | 33.34 (-1) | 7 (+1) | 0.06 (-1) | 19.54 | 16.55 |
7 | 30 (-1) | 300 (+1) | 7 (+1) | 0.34 (+1) | 54.45 | 51.46 |
8 | 30 (-1) | 33.34 (-1) | 3 (-1) | 0.06 (-1) | 32.84 | 35.66 |
9 | 30 (-1) | 166.67 (0) | 5 (0) | 0.2 (0) | 84.92 | 85.28 |
10 | 50 (+1) | 166.67 (0) | 5 (0) | 0.2 (0) | 86.87 | 87.23 |
11 | 40 (0) | 33.34 (-1) | 5 (0) | 0.2 (0) | 20.065 | 20.21 |
12 | 40 (0) | 300 (+1) | 5 (0) | 0.2 (0) | 88.61 | 88.97 |
13 | 40 (0) | 166.67 (0) | 3 (-1) | 0.2 (0) | 53.74 | 42.47 |
14 | 40 (0) | 166.67 (0) | 7 (+1) | 0.2 (0) | 36.69 | 48.67 |
15 | 40 (0) | 166.67 (0) | 5 (0) | 0.06 (-1) | 76.41 | 76.77 |
16 | 40 (0) | 166.67 (0) | 5 (0) | 0.34 (+1) | 42.25 | 42.61 |
17 | 40 (0) | 166.67 (0) | 5 (0) | 0.2 (0) | 62.45 | 65.97 |
18 | 40 (0) | 166.67 (0) | 5 (0) | 0.2 (0) | 76.22 | 65.97 |
19 | 40 (0) | 166.67 (0) | 5 (0) | 0.2 (0) | 66.51 | 65.97 |
20 | 40 (0) | 166.67 (0) | 5 (0) | 0.2 (0) | 64.78 | 65.97 |
21 | 40 (0) | 166.67 (0) | 5 (0) | 0.2 (0) | 62.02 | 65.97 |