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Figure 6 | BMC Biotechnology

Figure 6

From: Immobilized Rhizopus oryzaelipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology

Figure 6

(a) Thin layer chromatogram of palm stearin based, wax ester synthesis. Mobile phase: hexane/diethyl-ether/acetic acid 90:10:0.5 (v/v/v). Lane 1: mixture before reaction showing palm stearin (A) and cetyl alcohol (B); lane 2: mixture after 2 h of reaction time showing wax esters (C) and residual cetyl alcohol (D). (b) HPLC profiles of palm stearin/cetyl alcohol mixture obtained before at 0 h (1) and 2 h (2) of reaction times. The column used was: Lichrospher 100 RP-18. 5 μm (4 × 250 mm) and the mobile phase used was acetonitrile/acetone (50:50. v/v) with a flow rate of 1.5 mL/min. Temperature was maintained at 45°C and UV detection was at 206 nm.

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BMC Biotechnology

ISSN: 1472-6750

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