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Figure 4 | BMC Biotechnology

Figure 4

From: RNA interference in marine and freshwater sponges: actin knockdown in Tethya wilhelma and Ephydatia muelleriby ingested dsRNA expressing bacteria

Figure 4

Phalloidin-stained tissue in control vs. actin RNAi-treated Ephydatia muelleri. Confocal laser scanning microscopy images of BODIPY 558/568 phalloidin conjugate-stained whole-mount preparations of E. muelleri. A - C. Actin RNAi-treated sponge tissues. D - F. Control-treated sponges. A and D. Arrow shows Actin filament connections to substrate. These connections are generally missing in RNAi-treated tissues. B and E. Arrows show choanocyte chambers while arrowheads point to Actin connections (in endopinacocytes) between chambers, which are largely missing in treated sponges. C and F. Arrowheads show extensive Actin connections between and around choanocyte chambers. These connections are missing or not as extensive in treated tissues.

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BMC Biotechnology

ISSN: 1472-6750

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