From: A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillussp. SK37
Characteristic | Inhibitors | Concentration | Remaining activity (%) |
---|---|---|---|
Serine protease inhibitor | Leupeptin | 10 mM | 100.7 |
 | SBTI | 0.4 mg/ml | 100.9 |
 | TLCK | 10 mM | 99.4 |
 | TPCK | 10 mM | 99.9 |
 | PMSF | 1 mM | 89.2 |
 |  | 10 mM | 1.0 |
Metalloprotease inhibitor | EDTA | 0.5 mM | 78.1 |
 |  | 1 mM | 64.2 |
 |  | 5 mM | 1.4 |
 | Imidazole | 0.5 M | 82.4 |
 | L-Histidine | 0.5 M | 108.1 |
Cysteine protease inhibitor | IAA | 0.1 M | 98.0 |
 | NEM | 0.1 M | 114.2 |
 | E64 | 1 mM | 99.2 |
Aspartic protease inhibitor | Pepstatin A | 1 mM | 102.1 |
Amino peptidase inhibitor | Bestatin | 2 mM | 102.6 |
Denaturants | Substances | Concentration | Remaining activity (%) |
H-bond breaker | Urea | 1M | 20.8 |
 |  | 2M | 0.0 |
Detergent | SDS | 0.5% | 0.0 |
 |  | 1.0% | 0.0 |
Reducing agents | DTT | 1 mM | 38.5 |
 |  | 10 mM | 2.3 |
 |  | 20 mM | 0.1 |
 | BME | 1 mM | 64.7 |
 |  | 10 mM | 41.9 |
 |  | 20 mM | 14.1 |