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Figure 4 | BMC Biotechnology

Figure 4

From: A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillussp. SK37

Figure 4

SDS-PAGE and casein-zymography of recombinant AprX-SK37. Samples were electrophoresed on a 10-15% SDS polyacrylamide gel (lane 1 & 2) and 12% native polyacrylamide gel (lane 3 & 4). After electrophoresis, the gel was stained with Coomassie brilliant blue (lanes 1 - 3) or detected for protease activity (lane 4). Lane 1, whole cell lysate obtained from E. coli BL21 harbouring plasmid pET-AprX-SK37; lane 2, purified recombinant AprX-SK37; lane 3, purified recombinant AprX-SK37 run in a native polyacrylamide gel; lane 4, purified recombinant AprX-SK37 in casein-zymogram. Standard molecular weights for SDS-PAGE (lane 1 & 2) are marked by arrows.

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