From: Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein
Protein | Ligand | SPR | DSC | ||||
---|---|---|---|---|---|---|---|
ka(1/Ms) | kd(1/s) | KD(M) | Tm(°C) | ΔTm(°C) | ΔH ×104 (cal/mol) | ||
wtAvd | - | - | - | - | 85.5 | - | 5.4 |
wtAvd | Btn | n.d. | n.d. | n.d. | 123.2 | 37.7 | 12.8 |
wtAvd | Tes | - | - | no binding | 86.2 | 0.7 | 5.6 |
sbAvd-1 | - | - | - | - | 80.6 | - | 5.2 |
sbAvd-1 | Btn | 4.2 × 105 | 5.6 × 10-4 | 1.4 × 10-9 | 83.2 | 2.6 | 6.4 |
sbAvd-1 | Tes | 1.0 × 103 | 9.5 × 10-3 | 9.0 × 10-6 | 81.5 | 0.9 | 5.7 |
sbAvd-2 | - | - | - | - | 82.5 | - | 4.6 |
sbAvd-2 | Btn | 1.0 × 103 | 6.8 × 10-4 | 6.6 × 10-7 | 83.0 | 0.5 | 4.2 |
sbAvd-2 | Tes | 813 | 8.5 × 10-3 | 1.1 × 10-5 | 83.1 | 0.6 | 4.8 |