Figure 5
From: Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein
Inhibition analysis of sbAvd-1 and sbAvd-2 proteins by the SPR method. The binding of the sbAvd-1 and the sbAvd-2 to a CM5 sensor chip functionalized with testosterone-BSA was measured in the presence of 50 μM inhibitors. (A) The binding of the sbAvd-1 protein was totally inhibited by dehydroepiandrosterone, androstenedione, and biotin. (B) The binding of the sbAvd-2 protein was totally inhibited by dehydroepiandrosterone or testosterone, but not biotin. This result is due to the markedly decreased affinity of the protein towards biotin.
Samples: Protein sample, green; protein with estradiol, dark blue; protein with DHT, olive; protein with testosterone, black; protein with DHEAS, blue; protein with androstenedione, brown; protein with biotin, red.