Figure 4From: Modification of the loops in the ligand-binding site turns avidin into a steroid-binding proteinDetermination of ligand-binding specificity of sbAvd-1 and sbAvd-2 proteins by microplate analysis. The binding of sbAvd-1 and sbAvd-2 to a set of different small ligands was detected using polyclonal anti-avidin antibody as a probe (A) and the effect of free biotin (10 μM) to ligand-binding was analyzed (B).Abbreviations used in the figure: PBS, Phosphate buffered saline; HSA, human serum albumin; PRO, HSA-conjugated progesterone; HYD, HSA-conjugated hydrocortisone; TES, HSA-conjugated testosterone; CHO, HSA-conjugated cholic acid; TES-B, BSA-conjugated testosterone; BTN, BSA-conjugated D-biotin; BSA, bovine serum albumin.Back to article page