Figure 2
From: Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein
Immunoblot analysis of Avd phages with anti-Avd and anti-pIII antibodies. (A) The location of the Avd-pIII fusion protein recognized by anti-Avd is indicated by the upper arrowhead. The theoretical mass of the Avd-pIII fusion protein is 38 kDa. The produced free Avd (~14 kDa) is indicated with the lower arrowhead. (B) From the immunoblot analyzed with anti-pIII antibody the full-length pIII expressed from the helper phage (VCSM13) can be seen migrating at ~60 kDa (the upper arrowhead). The Avd-pIII fusion is indicated by the middle arrowhead. In addition, some proteolytically truncated Avd-pIII forms were detected (~33 kDa; lower arrowhead).
Lane 1: Avd-pIII phage; lane 2: Avd(N118M)-pIII phage; lane 3: Avd/Avd-pIII phage; lane 4: Avd(N118M)/Avd(N118M)-pIII phage. The molecular weights of standard proteins are shown as kilodaltons on the left side of each blot.