Figure 1
From: Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein
Schematic presentation of the Avd display expression constructs. (A) The phagemid constructs for Avd and Avd(N118M) display, in which Avd protein is produced solely as a fusion with pIII. (B) The phagemid constructs for Avd/Avd-pIII and Avd(N118M)/Avd(N118M)-pIII display, in which the pelB signal sequence is used for secretion of the Avd-pIII fusions and the free Avd. The cloning sites used are shown by vertical arrows.