Skip to main content

Table 1 Effect of preparation conditions on lipase immobilization efficiency and activity of lipase immobilized PD-MNP

From: Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating

Dopamine
(mg/ml)
PD-MNP
(mg)
Lipase (mg) Bound Lipase (mg) Activity
(U)
Specific Activity (U/mg lipase)
0 10 10 3.16 ± 0.52 9.75 ± 2.28 3.09 ± 0.16
0.25 10 10 6.96 ± 0.49 18.19 ± 2.90 2.62 ± 0.13
0.5 10 10 7.81 ± 0.62 21.08 ± 2.49 2.70 ± 0.17
1.0 10 10 8.26 ± 0.48 28.81 ± 3.08 3.49 ± 0.17
2.5 10 10 9.17 ± 0.58 59.00 ± 3.39 6.44 ± 0.38
3.75 10 10 9.37 ± 0.55 62.50 ± 3.04 6.67 ± 0.37
5.0 10 10 9.46 ± 0.57 64.89 ± 2.69 6.86 ± 0.40
2.5 10 5 4.29 ± 0.22 37.65 ± 2.52 8.78 ± 0.33
Free lipase   10   118.76 ± 4.13 11.88 ± 0.41
  1. Effect of preparation conditions on lipase immobilization efficiency and activity of lipase immobilized PD-MNP (n = 3).