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Figure 2 | BMC Biotechnology

Figure 2

From: Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques

Figure 2

Evaluation of DNA-Loading ratio (DLR). (A) Picture of ethidium bromide-stained agarose gel showing plasmid DNA-gold microcarrier coupling. The amount of microcarriers calculated to contain 100 ng of plasmid DNA was loaded per lane. Lanes: M, marker (1 Kb ladder; P, plasmid DNA (pCMV-LUC) alone; 0, 4, 10, 25 refer to DLRs for the respective lanes indicated. (B) Luciferase activity in mouse skin following biolistic transfer of pCMV-LUC at different DLRs. BLI was performed daily for four days after gene delivery (n = 6). (C-F) Representative pictures from different DLRs one day after biolistic gene transfer.

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