Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding

Figure 4

Detection of hPRR by ELISA. (A) Illustration of Detection of hPRR by ELISA. For determination of immobilized hPRR (left), purified hPRR were immobilized on a plate. And then, mouse anti-FLAG M2 antibody was incubated for binding hPRR and anti-FLAG M2 antibody. HRP-conjugated anti-mouse IgG antibody was used for detection of binding hPRR and anti-FLAG M2 antibody. Based on this hPRR calibration curve, the amount of displayed GFPuv-hPRR was determined (right). BmNPV-GFPuv-hPRR particles were immobilized on a plate. And then, mouse anti-FLAG M2 antibody was incubated for binding hPRR and anti-FLAG M2 antibody. Detection of hPRR was carried out by HRP-conjugated anti-mouse IgG antibody. (B) Calibration curve of purified hPRR by ELISA. (C) Detection of hPRR on the surface of BmNPV-GFPuv-hPRR particles. Closed circles and closed squares denote purified BmNPV GFPuv-hPRR particles and purified BmNPV-CP--GGT2 particles, respectively.

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us