Figure 5
From: Purification of phage display-modified bacteriophage T4 by affinity chromatography
Elution profile for the bacteriophage HAP1 modified with His tag and purified on Ni-NTA agarose compared to a non-modified phage. wash 1 - phage concentration in the washing flow-through (1st litre of washing buffer) wash 2 - phage concentration in the washing flow-through (2nd litre of washing buffer) wash 3 - phage concentration in the final washing fraction (3rd litre of washing buffer) elution 1 - phage concentration in the first elution fraction (imidazole 100 mM) elution 2 - phage concentration in the second elution fraction (imidazole 200 mM) elution 3 - phage concentration in the third elution fraction (imidazole 300 mM) elution 4 - phage concentration in the fourth elution fraction (imidazole 400 mM) elution 5 - phage concentration in the fifth elution fraction (imidazole 500 mM)