Figure 5

Screening of the hydrogen peroxide decomposing enzymes (MP-11, CAT or HRP). 3.05 mm graphite rod electrodes, -50 mV vs. Ag/AgCl, 1 μL of each enzymes and 1 μL of cell suspension. PDE and suspension of recombinant cells were entrapped behind the dialysis membrane (cut off 10 kDa). Testing solution: 30 mM Tris-HCl (pH 8.9); working volume: 20 mL; stock solution of uric acid: 10 mM in 30 mM Tris-HCl (pH 8.9).