Figure 3

Southern dot-blot of genomic DNAs of strains overproducing UOX probed with UOX ORF with terminator region. Genomic DNAs of strains A--24 and C--22 with following DNA dilution: 1-- 1 μg without dilution; 2--twofold dilution; 3--5-fold dilution; 4--10-fold dilution. B/1 and 4 -- 1 μg of genomic DNA of parental strain C-105 without dilution (one copy control). The labeling of probe DNA and hybridization was performed using ECL direct nucleic acid labeling and detection system.