Figure 1

Imaging of the microbubbles at the intramyocardial injection site. EGFP plasmid DNA (100 μg) was mixed with 0.1 ml of microbubble solution and injected into the myocardium of the left ventricle anterior wall. (a) Long-axis view before contrast injections. (b) The same long-axis view showing contrast injection at 5 min (indicated by the white arrow); the contrast is visible as the hyperintense signal indicated by the white arrow. (c) Long-axis view showing contrast injection at 10 min. (d) Long-axis view showing contrast injection at 20 min. (e) Long-axis view showing contrast injection at 30 min. (f) Long-axis view showing contrast injection at 40 min. (g) Long-axis view showing contrast injection at 50 min.(h) Long-axis view showing contrast injection at 60 min.