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Table 1 Comparison of different methods for purification of LVs.

From: Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration

 

SN

elution

concentrate

purification method

volume

[ml]

IP/ml

(± SEM)

IP

(± SEM)

volume

[ml]

IP/ml

(± SEM)

IP

(± SEM)

volume

[ml]

IP/ml

(± SEM)

IP

(± SEM)

MA/UF

(n = 7)

40

3.0 × 107

(± 4.7 × 106)

1.2 × 109

(± 1.8 × 108)

4.7

(± 0.2)

1.3 × 108

(± 2.2 × 107)

6.2 × 108

(± 9.5 × 107)

355

(± 32)

1.8 × 109

(± 4.0 × 108)

5.8 × 108

(± 1.2 × 108)

UC

(n = 3)

40

1.2 × 107

(± 1.3 × 106)

4.7 × 108

(4.8 ± × 107)

 

-

 

327

(± 18)

6.8 × 108

(± 8.9 × 107)

2.1 × 108

(± 2.1 × 107)

MA/UF

(n = 6)

500

1.4 × 107

(± 8.6 × 105)

7.1 × 109

(± 5.2 × 108)

25.8

(± 0.2)

1.5 × 108

(± 3.5 × 107)

2.7 × 109

(± 3.5 × 108)

1064

(± 118)

2.7 × 109

(± 3.7 × 108)

2.8 × 109

(± 5.0 × 108)

UC

(n = 3)

500

2.5 × 107

(± 6.6 × 106)

1.3 × 1010

(± 3.3 × 109)

 

-

 

776

(± 152)

6.1 × 109

(± 1.1 × 109)

4.6 × 109

(± 1.1 × 109)

MA/UF

(n = 3)

1000

1.8 × 107

(± 4.9 × 106)

1.9 × 1010

(± 5.2 × 109)

40.6

(± 0.7)

1.7 × 108

(± 4.8 × 107)

7.0 × 109

(± 1.9 × 109)

1510

(± 220)

5.4 × 109

(± 1.8 × 109)

8.6 × 109

(± 3.6 × 109)

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BMC Biotechnology

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