Purification of LVs from different starting volumes. LVs were purified from 40 ml cell culture SN by using the small MA/UF setup (A) or UC (B); from 500 ml of starting volume by application of the large MA/UF combination (C) or UC (D) and from 1,000 ml of cell culture SN and use of two serially connected large MAs and UF (E). IP/ml were determined via FACS-analysis and the recovery rate (%) was calculated. n = 7 (A), n = 3 (B); n = 6 (C), n = 3 (D), n = 3 (E). mean + SEM.