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Table 3 Proof-of-concept results for the Brachypodium hormone and photoperiod libraries

From: Construction of high quality Gateway™ entry libraries and their application to yeast two-hybrid for the monocot model plant Brachypodium distachyon

  Photoperiod Library Hormone Library
Interactors # Isolated Percent # Isolated Percent
NF-YB Family     
   Bradi1g21900 17 23 12 19
   Bradi2g54200 5 7 8 13
   Bradi3g15670 0 0 19 31
CCT Family     
   Bradi1g11310 11 15 0 0
   Bradi2g14220 6 8 0 0
   Bradi3g05800 1 1 1 2
   Bradi3g33340 3 4 0 0
   Bradi3g41500 *11 15 0 0
   Bradi5g14600 5 7 0 0
Novel Interactors 14 19 22 35
Overall Totals 73 100 62 100
  1. *6/11 blast hits for Bradi3g415000 were short (25-60bp) matches, but the other five were long matches (~380 bp perfect match).
  2. Annotations as NF-YB or CCT proteins came from direct nucleotide blasts (blastn) of Y2H Bradi3g05270 interactors at the Brachypodium database (http://www.brachypodium.org) and/or blastx at The Arabidopsis Information Resource (TAIR, http://www.arabidopsis.org).