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Table 3 Proof-of-concept results for the Brachypodium hormone and photoperiod libraries

From: Construction of high quality Gateway™ entry libraries and their application to yeast two-hybrid for the monocot model plant Brachypodium distachyon

 

Photoperiod Library

Hormone Library

Interactors

# Isolated

Percent

# Isolated

Percent

NF-YB Family

    

   Bradi1g21900

17

23

12

19

   Bradi2g54200

5

7

8

13

   Bradi3g15670

0

0

19

31

CCT Family

    

   Bradi1g11310

11

15

0

0

   Bradi2g14220

6

8

0

0

   Bradi3g05800

1

1

1

2

   Bradi3g33340

3

4

0

0

   Bradi3g41500

*11

15

0

0

   Bradi5g14600

5

7

0

0

Novel Interactors

14

19

22

35

Overall Totals

73

100

62

100

  1. *6/11 blast hits for Bradi3g415000 were short (25-60bp) matches, but the other five were long matches (~380 bp perfect match).
  2. Annotations as NF-YB or CCT proteins came from direct nucleotide blasts (blastn) of Y2H Bradi3g05270 interactors at the Brachypodium database (http://www.brachypodium.org) and/or blastx at The Arabidopsis Information Resource (TAIR, http://www.arabidopsis.org).
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BMC Biotechnology

ISSN: 1472-6750

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