Figure 5
From: Identification of a novel temperature sensitive promoter in cho cells
Luciferase activity of S100a6 promoter constructs. 5 different constructs of the S100a6 promoter in series 4 compared to dS16, +C (pGL3-P, the positive control) and -C (pGL3-B, the negative control). Each construct was co-transfected with pRL-SV40 into CHO cells. Cell suspensions were measured for luciferase activity 24 hours after transfection and incubation at 33°C. All relative luciferase activities were normalized to Renilla luciferase activity. The average of two independent experiments with triplicate samples is shown for each construct.