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Table 1 Effects of restriction endonucleases (50 copies/assay).

From: Multiple strategies to improve sensitivity, speed and robustness of isothermal nucleic acid amplification for rapid pathogen detection

 

Tt (Min)

Min Tt (Min)

Max Tt (Min)

Max Difference

of Tt (Min)

With MboI

26.88 ± 0.88

25.20

28.00

2.80

Without MboI

32.30 ± 2.70

29.02

37.84

8.82