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Figure 4 | BMC Biotechnology

Figure 4

From: Potential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensor

Figure 4

Online signals of dissolved oxygen tension (DOT) and oxygen transfer rate (OTR) in a cultivation of E. coli BL21 pET28a in TB medium; experimental conditions: temperature T = 37°C, shaking diameter d0 = 25 mm, shaking frequency n = 300 rpm, filling volume VL = 25 ml, initial optical density ODα = 0.3; (dashed line) DOT measured with a sensor spot fixed to the inside flask wall, (full line) DOT measured with rotating flexitube optical sensor system according to Fig. 2, (circle) OTR in flask without flexitube optical sensor, (triangle) OTR in flask with flexitube optical sensor.

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