Figure 3From: Potential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensorOnline signals of dissolved oxygen tension (DOT) and oxygen transfer rate (OTR) in a cultivation of E. coli BL21 pET28a in TB medium at different shaking frequencies. DOT was measured with sensor spot fixed to the inside flask wall (refer to Fig. 2A); experimental conditions: temperature T = 37°C, shaking diameter d0 = 50 mm, filling volume VL = 25 ml, initial optical density ODα = 0.3.Back to article page