Figure 6
From: Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris
Integration of the pPICZB vector into the P. pastoris genome. Schematic (adapted from the Invitrogen EasySelect Pichia expression kit manual) of the integration of the pPICZB vector into the P. pastoris genome by gene insertion. Double headed arrows indicate the sequence that was amplified using the PpAOX1 TT primer pair when quantifying incorporated plasmids by qPCR. To obtain the gene of interest (GOI) copy number (n), the AOX1 TT copy number was subtracted by 1 to compensate for the endogenous AOX1 TT sequence.