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Table 2 Primer sequences used in the RQ-PCR experiments

From: mRNA transcript quantification in archival samples using multiplexed, color-coded probes

Gene symbol

Primer sequence

Amplicon length

GAPDH

Forward 5'-CCTGTTCGACAGTCAGCCGCAT-3'

Reverse 5'-GACTCCGACCTTCACCTTCCCC-3'

87 bp

RPS18

Forward 5'-GCGGCGGAAAATAGCCTTTGCC-3'

Reverse 5'-CCTCTTGGTGAGGTCAATGTCTGC-3'

100 bp

MMP1

Forward 5'-CAAATGGGCTTGAAGCTGCTTACG-3'

Reverse 5'-GTGTAGCACATTCTGTCCCTGAACA-3'

101 bp

COL4A1

Forward 5'-AAGGACCAAAAGGTGACAAGGGTGA-3'

Reverse 5'-GAACTTGAGCTTGTCCTGGTACTCC-3'

72 bp

COL5A1

Forward 5'-GTCATCCCACCATCACCAAAGCC-3'

Reverse 5'-ATCGGGAAGGCACCTGCAGAATG-3'

92 bp

THBS2

Forward 5'-TTGCAAATGGGTGTGACGCGGT-3'

Reverse 5'-AAGCACCGCACTTTGCTCTGCT-3'

86 bp

TNC

Forward 5'-ACGAACACTCAATCCAGTTTGCTGA-3'

Reverse 5'-TGGAATTTATGCCCGTTTGCGCC-3'

89 bp

COL3A1

Forward 5'-TGGCACAACAGGAAGCTGTTGAAGG-3'

Reverse 5'-ACACATATTTGGCATGGTTCTGGCT-3'

97 bp

COL5A2

Forward 5'-TCATGCTACCCTGAAGTCACTCAGT-3'

Reverse 5'-AGGTCATCACACGTGCGGGC-3'

93 bp

PDPN

Forward 5'-CAGGAACCAGCGAAGACCGCT-3'

Reverse 5'-TGGCAGATCCTCGATGCGAATGC-3'

95 bp

POSTN

Forward 5'-CGGTCACTTCACACTCTTTGCTCCC-3'

Reverse 5'-CGGAAGCCACTTTGTCTCCCATGA-3'

95 bp

SERPINE2

Forward 5'-ACCATGAACTGGCATCTCCCCCT-3'

Reverse 5'-TGGAGCCTAGTTCCTCGAGAGACA-3'

100 bp

SERPINH1

Forward 5'-CCGTGGCTTCATGGTGACTCGG-3'

Reverse 5'-AGTAGTTGTAGAGGCCTGTCCGGT-3'

74 bp

SDHA

Forward 5'-CTCCAAGCCCATCCAGGGGCAA-3'

Reverse 5'-CAGAGTGACCTTCCCAGTGCCAA-3'

100 bp

PLOD2

Forward 5'-TGGCTCTTTGCCGAAATGCTAGAG-3'

Reverse 5'-GGGGGCTGAGCATTTGGAATGTTT-3'

87 bp

P4HA2

Forward: 5'-AGGAGCTGCCAAAGCCCTGA-3'

Reverse: 5'-ACCTGCTCCATCCACAACACCG-3'

170 bp

CTHRC1

Forward: 5'-TTGTTCAGTGGCTCACTTCG-3'

Reverse: 5'-TTCAATGGGAAGAGGTCCTG-3'

102 bp

CXCL1

Forward: 5'-ATTTCTGAGGAGCCTGCAAC-3'

Reverse: 5'-CACATACATTCCCCTGCCTT-3'

100 bp

CXCL13

Forward: 5'-GAGCCTGTCAAGAGGCAAAG-3'

Reverse: 5'-CTGGGGATCTTCGAATGCTA-3'

142 bp

  1. SERPINE1 was excluded from RQ-PCR analysis since no primer pairs tested showed good efficiency for amplification in FFPE samples.
  2. Primer sequences used yielded short amplicon lengths, as indicated.