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Table 2 Primer sequences used in the RQ-PCR experiments

From: mRNA transcript quantification in archival samples using multiplexed, color-coded probes

Gene symbol Primer sequence Amplicon length
GAPDH Forward 5'-CCTGTTCGACAGTCAGCCGCAT-3'
Reverse 5'-GACTCCGACCTTCACCTTCCCC-3'
87 bp
RPS18 Forward 5'-GCGGCGGAAAATAGCCTTTGCC-3'
Reverse 5'-CCTCTTGGTGAGGTCAATGTCTGC-3'
100 bp
MMP1 Forward 5'-CAAATGGGCTTGAAGCTGCTTACG-3'
Reverse 5'-GTGTAGCACATTCTGTCCCTGAACA-3'
101 bp
COL4A1 Forward 5'-AAGGACCAAAAGGTGACAAGGGTGA-3'
Reverse 5'-GAACTTGAGCTTGTCCTGGTACTCC-3'
72 bp
COL5A1 Forward 5'-GTCATCCCACCATCACCAAAGCC-3'
Reverse 5'-ATCGGGAAGGCACCTGCAGAATG-3'
92 bp
THBS2 Forward 5'-TTGCAAATGGGTGTGACGCGGT-3'
Reverse 5'-AAGCACCGCACTTTGCTCTGCT-3'
86 bp
TNC Forward 5'-ACGAACACTCAATCCAGTTTGCTGA-3'
Reverse 5'-TGGAATTTATGCCCGTTTGCGCC-3'
89 bp
COL3A1 Forward 5'-TGGCACAACAGGAAGCTGTTGAAGG-3'
Reverse 5'-ACACATATTTGGCATGGTTCTGGCT-3'
97 bp
COL5A2 Forward 5'-TCATGCTACCCTGAAGTCACTCAGT-3'
Reverse 5'-AGGTCATCACACGTGCGGGC-3'
93 bp
PDPN Forward 5'-CAGGAACCAGCGAAGACCGCT-3'
Reverse 5'-TGGCAGATCCTCGATGCGAATGC-3'
95 bp
POSTN Forward 5'-CGGTCACTTCACACTCTTTGCTCCC-3'
Reverse 5'-CGGAAGCCACTTTGTCTCCCATGA-3'
95 bp
SERPINE2 Forward 5'-ACCATGAACTGGCATCTCCCCCT-3'
Reverse 5'-TGGAGCCTAGTTCCTCGAGAGACA-3'
100 bp
SERPINH1 Forward 5'-CCGTGGCTTCATGGTGACTCGG-3'
Reverse 5'-AGTAGTTGTAGAGGCCTGTCCGGT-3'
74 bp
SDHA Forward 5'-CTCCAAGCCCATCCAGGGGCAA-3'
Reverse 5'-CAGAGTGACCTTCCCAGTGCCAA-3'
100 bp
PLOD2 Forward 5'-TGGCTCTTTGCCGAAATGCTAGAG-3'
Reverse 5'-GGGGGCTGAGCATTTGGAATGTTT-3'
87 bp
P4HA2 Forward: 5'-AGGAGCTGCCAAAGCCCTGA-3'
Reverse: 5'-ACCTGCTCCATCCACAACACCG-3'
170 bp
CTHRC1 Forward: 5'-TTGTTCAGTGGCTCACTTCG-3'
Reverse: 5'-TTCAATGGGAAGAGGTCCTG-3'
102 bp
CXCL1 Forward: 5'-ATTTCTGAGGAGCCTGCAAC-3'
Reverse: 5'-CACATACATTCCCCTGCCTT-3'
100 bp
CXCL13 Forward: 5'-GAGCCTGTCAAGAGGCAAAG-3'
Reverse: 5'-CTGGGGATCTTCGAATGCTA-3'
142 bp
  1. SERPINE1 was excluded from RQ-PCR analysis since no primer pairs tested showed good efficiency for amplification in FFPE samples.
  2. Primer sequences used yielded short amplicon lengths, as indicated.