Structure of the plasmids and the germinated pea seeds ready for agroinfection. (a) pCAPE1 containing full-length cDNA of PEBV RNA-1 with an intron. (b) pCAPE2-GFP containing cDNA of PEBV RNA-2 with the GFP coding sequence replacing the genes required for nematode transmission. CP is the coat protein coding region. (c) The 36-48 hours germinated pea seeds with 2-3 cm roots were vacuum infiltrated with cultures of Agrobacterium containing binary plasmids pCAPE1and pCAPE2-GFP or pCAPE1 and pCAPE2-aFGF.