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Table 2 Relative affinity of a single candidate biotin derivative in its mixture sample prepared via PCS.

From: Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model

compounds Relative affinity Relatively affinity viaEq.(6)
  Homogenous method Fine optimizationa Rough optimizationb
BMPL 1.00 1.00
BCHA 3.68 ± 0.54 (5) 3.13 ± 0.27 (6) 3.23 ± 0.12 (2)
BME 1.90 ± 0.34 (2) 1.85 ± 0.25 (6) 1.65 ± 0.15 (2)
BDETA 0.45 ± 0.06 (4) 0.41 ± 0.11 (6) 0.51 ± 0.05 (2)
  1. Number in parenthesis was for independent assays. For each candidate biotin derivative, different methods yielded consistent relative affinities.
  2. a The average of the stable relative affinities on the response curve.
  3. b The result was from the second PMFS containing the approximated candidate biotin derivative at 5.0 μM.