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Table 2 Relative affinity of a single candidate biotin derivative in its mixture sample prepared via PCS.

From: Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model

compounds

Relative affinity

Relatively affinity viaEq.(6)

 

Homogenous method

Fine optimizationa

Rough optimizationb

BMPL

1.00

1.00

BCHA

3.68 ± 0.54 (5)

3.13 ± 0.27 (6)

3.23 ± 0.12 (2)

BME

1.90 ± 0.34 (2)

1.85 ± 0.25 (6)

1.65 ± 0.15 (2)

BDETA

0.45 ± 0.06 (4)

0.41 ± 0.11 (6)

0.51 ± 0.05 (2)

  1. Number in parenthesis was for independent assays. For each candidate biotin derivative, different methods yielded consistent relative affinities.
  2. a The average of the stable relative affinities on the response curve.
  3. b The result was from the second PMFS containing the approximated candidate biotin derivative at 5.0 μM.
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BMC Biotechnology

ISSN: 1472-6750

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