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Table 5 Primer Pairs Employed for the RT-PCR Amplification of Native Biosynthetic Genes AaPMT and AaTRI

From: Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots

Primer Pairs KF ofAaPMT, AaTRI (Forward) and 3'UTR ofAaPMT, AaTRI

AaPMT

Forward: 5'-ATGGAGGTCATAAGCAACCAC-3'

3-UTR 156R: 5'-ATATCAGTTTATTGCATTATAC-3'

AaTRI

Forward: 5'-ATGGGAGAATCAAAAGTTTACAT-3'

3-UTR 244R:5'-TTGCGACATTTTATTGTGATGA-3'