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Table 4 Primer Pairs Employed for the RT-PCR Amplification of Total Biosynthetic Genes AaPMT and AaTRI

From: Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots

Primer Pairs: KF ofAaPMT, AaTRI(Forward) and KR ofAaPMT, AaTRI(Reverse)

AaPMT

Forward: 5'-ATGGAGGTCATAAGCAACCAC-3'

Reverse: 5'-TCAAAATTCAACCAAATCCC-3'

AaTRI

Forward: 5'-ATGGGAGAATCAAAAGTTTACAT-3'

Reverse: 5'-TCAAAATCCACCATTAGCTGTGA-3'

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