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Table 3 Primer Pairs Employed for the PCR Amplification of TA Biosynthetic Genes AaPMT and AaTRI

From: Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots

Primer Pairs 35S PROM 252F(Forward)and KR ofAaPMT andAaTRI(Reverse)

AaPMT

Forward: 5'-ATGGAGGTCATAAGCAACCAC-3'

Reverse: 5'-TCAAAATTCAACCAAATCCC-3'

AaTRI

Forward: 5'-ATGGGAGAATCAAAAGTTTACAT-3'

Reverse: 5'-TCAAAATCCACCATTAGCTGTGA-3'

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BMC Biotechnology

ISSN: 1472-6750

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